Cell-selective metabolic labeling of proteins
نویسندگان
چکیده
منابع مشابه
Cell-selective metabolic labeling of proteins.
Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in ...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2009
ISSN: 1552-4450,1552-4469
DOI: 10.1038/nchembio.200